Zur Seitenansicht
 

Titelaufnahme

Titel
The NMR signature of gluconoylation: a frequent N-terminal modification of isotope-labeled proteins
VerfasserSchweida, David ; Barraud, Pierre ; Regl, Christof ; Loughlin, Fionna E. ; Huber, Christian G. ; Cabrele, Chiara ; Schubert, Mario
Erschienen in
Journal of Biomolecular NMR, Amsterdam, 2019, Jg. 2019, S. 1-9
ErschienenAmsterdam : Springer Netherlands, 2019
SpracheEnglisch
DokumenttypAufsatz in einer Zeitschrift
Schlagwörter (EN)NMR spectroscopy / Post-translational protein modification / Gluconoylation / Gluconic acid / N-terminus / Recombinant protein / E. coli
ISSN0925-2738
URNurn:nbn:at:at-ubs:3-11293 Persistent Identifier (URN)
DOI10.1007/s10858-019-00228-6 
Zugriffsbeschränkung
 Das Werk ist frei verfügbar
Dateien
The NMR signature of gluconoylation: a frequent N-terminal modification of isotope-labeled proteins [2.95 mb]
Links
Nachweis
Klassifikation
Zusammenfassung (Englisch)

N-terminal gluconoylation is a moderately widespread modification in recombinant proteins expressed in Escherichia coli, in particular in proteins bearing an N-terminal histidine-tag. This post-translational modification has been investigated mainly by mass spectrometry. Although its NMR signals must have been observed earlier in spectra of 13C/15N labeled proteins, their chemical shifts were not yet reported. Here we present the complete 1H and 13C chemical shift assignment of the N-terminal gluconoyl post-translational modification, based on a selection of His-tagged protein constructs (CCL2, hnRNP A1 and Lin28) starting with Met-Gly-...-(His)6. In addition, we show that the modification can hydrolyze over time, resulting in a free N-terminus and gluconate. This leads to the disappearance of the gluconoyl signals and the appearance of gluconate signals during the NMR measurements. The chemical shifts presented here can now be used as a reference for the identification of gluconoylation in recombinant proteins, in particular when isotopically labeled.

Statistik
Das PDF-Dokument wurde 2 mal heruntergeladen.
Lizenz
CC-BY-Lizenz (4.0)Creative Commons Namensnennung 4.0 International Lizenz