Zur Seitenansicht
 

Titelaufnahme

Titel
Comparing proteolytic fingerprints of antigen-presenting cells during allergen processing
VerfasserHofer, Heidi ; Weidinger, Tamara ; Briza, Peter ; Asam, Claudia ; Wolf, Martin ; Twaroch, Teresa E. ; Stolz, Frank ; Neubauer, Angela ; Dall, Elfriede ; Hammerl, Peter ; Jacquet, Alain ; Wallner, Michael
Erschienen in
International Journal of Molecular Sciences, Basel, 2017, Jg. 18, H. 6, S. 1-12
ErschienenMDPI, 2017
SpracheEnglisch
DokumenttypAufsatz in einer Zeitschrift
Schlagwörter (EN)allergen proteolysis / Bet v 1 / Amb a 1 / Der p 1 / Der p 2 / proteases from dendritic cells / proteases from macrophages / proteases from B cells / degradome assay
ISSN1422-0067
URNurn:nbn:at:at-ubs:3-5638 Persistent Identifier (URN)
DOI10.3390/ijms18061225 
Zugriffsbeschränkung
 Das Werk ist frei verfügbar
Dateien
Comparing proteolytic fingerprints of antigen-presenting cells during allergen processing [4.78 mb]
Links
Nachweis
Klassifikation
Zusammenfassung (Englisch)

<span>Endolysosomal processing has a critical influence on immunogenicity as well as immune polarization of protein</span><span class="searchterm">antigens</span><span>. In industrialized countries, allergies affect around 25% of the population. For the rational design of protein-based allergy therapeutics for immunotherapy, a good knowledge of T cell-reactive regions on allergens is required. Thus, we sought to analyze endolysosomal degradation patterns of inhalant allergens. Four major allergens from ragweed, birch, as well as house dust mites were produced as recombinant proteins. Endolysosomal proteases were purified by differential centrifugation from dendritic cells, macrophages, and B cells, and combined with allergens for</span><span class="searchterm">proteolytic</span><span>processing. Thereafter, endolysosomal proteolysis was monitored by protein gel electrophoresis and mass spectrometry. We found that the overall</span><span class="searchterm">proteolytic</span><span>activity of specific endolysosomal fractions differed substantially, whereas the degradation patterns of the four model allergens obtained with the different proteases were extremely similar. Moreover, previously identified T cell epitopes were assigned to endolysosomal peptides and indeed showed a good overlap with known T cell epitopes for all four candidate allergens. Thus, we propose that the degradome assay can be used as a predictor to determine</span><span class="searchterm">antigenic</span><span>peptides as potential T cell epitopes, which will help in the rational design of protein-based allergy vaccine candidates.</span>

Statistik
Das PDF-Dokument wurde 14 mal heruntergeladen.
Lizenz
CC-BY-Lizenz (4.0)Creative Commons Namensnennung 4.0 International Lizenz