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Title
Prophylactic mRNA vaccination against allergy confers long-term memory responses and persistent protection in mice
AuthorHattinger, E. ; Scheiblhofer, S. ; Roesler, E. ; Thalhamer, T. ; Weiss, R.
Published in
Journal of Immunology Research, Kairo, 2015, Vol. 2015, Issue Article ID 797421, page 1-12
PublishedHindawi, 2015
LanguageEnglish
Document typeJournal Article
ISSN1550-6606
URNurn:nbn:at:at-ubs:3-3466 Persistent Identifier (URN)
DOI10.1155/2015/797421 
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 The work is publicly available
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Prophylactic mRNA vaccination against allergy confers long-term memory responses and persistent protection in mice [1.88 mb]
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Abstract (English)

Recently, mRNA vaccines have been introduced as a safety-optimized alternative to plasmid DNA-based vaccines for protection against allergy. However, it remained unclear whether the short persistence of this vaccine type would limit memory responses and whether the protective immune response type would be maintained during recurrent exposure to allergen. We tested the duration of protective memory responses in mice vaccinated with mRNA encoding the grass pollen allergen Phl p 5 by challenging them with recombinant allergen, 3.5, 6, and 9 months after vaccination. In a second experiment, vaccinated mice were repeatedly challenged monthly with aerosolized allergen over a period of 7 months. Antibody and cytokine responses as well as lung inflammation and airway hyperresponsiveness were assessed. mRNA vaccination induced robust TH1 memory responses for at least 9 months. Vaccination efficiently suppressed TH2 cytokines, IgE responses, and lung eosinophilia. Protection was maintained after repeated exposure to aerosolized allergen and no TH1 associated pathology was observed. Lung function remained improved compared to nonvaccinated controls. Our data clearly indicate that mRNA vaccination against Phl p 5 induces robust, long-lived memory responses, which can be recalled by allergen exposure without side effects. mRNA vaccines fulfill the requirements for safe prophylactic vaccination without the need for booster immunizations.