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Title
Cell wall ingrowths in nematode induced syncytia require UGD2 and UGD3
AuthorSiddique, Shahid ; Sobczak, Miroslaw ; Tenhaken, Raimund ; Grundler, Florian M. W. ; Bohlmann, Holger
Published in
PLOS One, Lawrence, 2012, Vol. 7, page 1-12
PublishedPublic Library of Science, 2012
LanguageEnglish
Document typeJournal Article
Keywords (EN)Plant cell walls / Nematode infections / Arabidopsis thaliana / Gene expression / Cytoplasm / Cellular structures and organelles / Polysaccharides / Seedlings
Project-/ReportnumberP16296-B06
Project-/ReportnumberP20471-B11
ISSN1932-6203
URNurn:nbn:at:at-ubs:3-1868 Persistent Identifier (URN)
DOI10.1371/journal.pone.0041515 
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 The work is publicly available
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Cell wall ingrowths in nematode induced syncytia require UGD2 and UGD3 [0.7 mb]
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Abstract (English)

The cyst nematode Heterodera schachtii infects roots of Arabidopsis plants and establishes feeding sites called syncytia, which are the only nutrient source for nematodes. Development of syncytia is accompanied by changes in cell wall structures including the development of cell wall ingrowths. UDP-glucuronic acid is a precursor of several cell wall polysaccharides and can be produced by UDP-glucose dehydrogenase through oxidation of UDP-glucose. Four genes in Arabidopsis encode this enzyme. Promoter::GUS analysis revealed that UGD2 and UGD3 were expressed in syncytia as early as 1 dpi while expression of UGD1 and UGD4 could only be detected starting at 2 dpi. Infection assays showed no differences between ugd1 and ugd4 single mutants and wild type plants concerning numbers of males and females and the size of syncytia and cysts. On single mutants of ugd2 and ugd3, however, less and smaller females, and smaller syncytia formed compared to wild type plants. The double mutant ugd23 had a stronger effect than the single mutants. These data indicate that UGD2 and UGD3 but not UGD1 and UGD4 are important for syncytium development. We therefore studied the ultrastructure of syncytia in the ugd23 double mutant. Syncytia contained an electron translucent cytoplasm with degenerated cellular organelles and numerous small vacuoles instead of the dense cytoplasm as in syncytia developing in wild type roots. Typical cell wall ingrowths were missing in the ugd23 double mutant. Therefore we conclude that UGD2 and UGD3 are needed for the production of cell wall ingrowths in syncytia and that their lack leads to a reduced host suitability for H. schachtii resulting in smaller syncytia, lower number of developing nematodes, and smaller females.

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CC-BY-License (4.0)Creative Commons Attribution 4.0 International License