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The fold variant BM4 Is beneficial in a therapeutic bet v 1 mouse model
AuthorPichler, Ulrike ; Asam, Claudia ; Weiss, Richard ; Isakovic, Almedina ; Hauser, Michael ; Briza, Peter ; Ferreira, Fátima In der Gemeinsamen Normdatei der DNB nachschlagen ; Wallner, Michael
Published in
BioMed Research International, Kairo, 2013, Vol. 2013, Issue Article ID 832404, page 1-5
PublishedHindawi, 2013
Document typeJournal Article
URNurn:nbn:at:at-ubs:3-260 Persistent Identifier (URN)
 The work is publicly available
The fold variant BM4 Is beneficial in a therapeutic bet v 1 mouse model [1.43 mb]
Abstract (English)

Background. Specific immunotherapy using recombinant allergens is clinically effective; still wild-type allergens can provoke treatment-induced side effects and often show poor immunogenicity in vivo. Thus, we tested the low IgE-binding, highly immunogenic fold variant BM4 in a Bet v 1 mouse model. Methods. Recombinant BM4 was used as active vaccine ingredient to treat mice sensitized to Bet v 1. As controls, mice were treated with either Bet v 1 or sham, and the humoral as well as cellular immune response was monitored. Moreover, lung function and lung inflammation were analysed. Results. BM4 was more effective than wild-type Bet v 1 in inducing Bet v 1-specific blocking antibodies as well as IFN- and IL-10 producing T cells. Further, birch pollen induced lung inflammation could be ameliorated significantly by BM4 treatment as demonstrated by a reduction of airway hyperresponsiveness and drastically decreased eosinophil counts in bronchoalveolar lavage fluids. Conclusion. The study outlines the high potential of BM4 as vaccine candidate for the treatment of Bet v 1-mediated birch pollen allergies.