Go to page
 

Bibliographic Metadata

Title
A comprehensive tutorial on In Vitro characterization of new photosensitizers for photodynamic antitumor therapy and photodynamic inactivation of microorganisms
AuthorKiesslich, Tobias ; Gollmer, Anita ; Maisch, Tim In der Gemeinsamen Normdatei der DNB nachschlagen ; Berneburg, Mark In der Gemeinsamen Normdatei der DNB nachschlagen ; Plätzer, Kristjan In der Gemeinsamen Normdatei der DNB nachschlagen
Published in
BioMed Research International, Kairo, 2013, Vol. 2013, Issue Article ID 840417, page 1-17
Published2013
LanguageEnglish
Document typeJournal Article
URNurn:nbn:at:at-ubs:3-235 Persistent Identifier (URN)
Restriction-Information
 The work is publicly available
Files
A comprehensive tutorial on In Vitro characterization of new photosensitizers for photodynamic antitumor therapy and photodynamic inactivation of microorganisms [2.49 mb]
Links
Reference
Classification
Abstract (English)

In vitro research performed on eukaryotic or prokaryotic cell cultures usually represents the initial step for characterization of a novel photosensitizer (PS) intended for application in photodynamic therapy (PDT) of cancer or photodynamic inactivation (PDI) of microorganisms. Although many experimental steps of PS testing make use of the wide spectrum of methods readily employed in cell biology, special aspects of working with photoactive substances, such as the autofluorescence of the PS molecule or the requirement of light protection, need to be considered when performing in vitro experiments in PDT/PDI. This tutorial represents a comprehensive collection of operative instructions, by which, based on photochemical and photophysical properties of a PS, its uptake into cells, the intracellular localization and photodynamic action in both tumor cells and microorganisms novel photoactive molecules may be characterized for their suitability for PDT/PDI. Furthermore, it shall stimulate the efforts to expand the convincing benefits of photodynamic therapy and photodynamic inactivation within both established and new fields of applications and motivate scientists of all disciplines to get involved in photodynamic research.